Coding

Part:BBa_K112119:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


rbs.Cre!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR mv030/mv002 on pBca9145-Bca1010    (1082bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112119
-----------------------------------------------
mv030	Forward Biobricking of rbs.Cre!
cgataGAATTCatgAGATCTaccattatctgagtgttaaatgtccaatttactgaccgtacacc
mv002	Reverse Biobricking of <Cre!
cgttaGGATCCttaatcgccatcttccagc

References